Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

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The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic g5210t-p90 Y.enterocolitica in animal and food samples.In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene.Additionally, biochemical tests 6-0 igora vibrance and RT-PCR showed that nine Y.enterocolitica isolates carried the ail-gene.

Two Y.enterocolitica isolates of 1A biotype had the ail gene.The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the ail gene Y.enterocolitica in the material examined.